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Chat Online· The tissue was ground in 5–6 ml of the grinding buffer (0.1 M phosphate buffer pH 7.5 containing 0.5 mM EDTA) for assaying antioxidant enzyme activities. The homogenate was centrifuged at 15 000 rpm at 4 °C for 20 min and the enzyme extract was assayed for
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Chat Online· The tissue was ground in 5–6 ml of the grinding buffer (0.1 M phosphate buffer pH 7.5 containing 0.5 mM EDTA) for assaying antioxidant enzyme activities. The homogenate was centrifuged at 15 000 rpm at 4 °C for 20 min and the enzyme extract was assayed for
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